mouse anti rat glut4 mab  (Cell Signaling Technology Inc)

Journal: Heliyon

Article Title: Effects of Salvia mirzayanii extract administration on hyperglycemia improvement in diabetic rats: The role of GLUT4, PEPCK and G6Pase genes

doi: 10.1016/j.heliyon.2024.e25256

Figure Lengend Snippet: Primer sequences and expected ampliocn size of the genes for RT-qPCR analysis.

Article Snippet: Slides were then treated with mouse anti-rat GLUT4 mAb (Cell Signaling Technology, USA), anti-rat G6Pase polyclonal antibody (Biorbyt Ltd, United Kingdom) and mouse anti-rat PEPCK mAb (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a 10 μg/mL concentration.

Techniques: Sequencing

Journal: Heliyon

Article Title: Effects of Salvia mirzayanii extract administration on hyperglycemia improvement in diabetic rats: The role of GLUT4, PEPCK and G6Pase genes

doi: 10.1016/j.heliyon.2024.e25256

Figure Lengend Snippet: Analysis of GLUT4 expression in the muscle tissue in normal control (NC), diabetic control (DC) and diabetic control treated with S. mirzayanii (DC-Treated with SM). (A) Analysis of mRNA expression using RT-qPCR. Data were normalized with that of β-actin and then calculated as relative to the NC group. Each value represents the mean ± SEM (n = 10 per group) in two independent experiments. P < 0.01: Significant difference vs. control group. (B) Protein expression level analysis by IHC technique in NC, DC and DC-Treated with SM. The small white arrows indicate the expression of GLUT4 in the muscle tissue.

Article Snippet: Slides were then treated with mouse anti-rat GLUT4 mAb (Cell Signaling Technology, USA), anti-rat G6Pase polyclonal antibody (Biorbyt Ltd, United Kingdom) and mouse anti-rat PEPCK mAb (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a 10 μg/mL concentration.

Techniques: Expressing, Control, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: The Effect of Gonadotropin on Glucose Transport and Apoptosis in Rat Ovary

doi: 10.1371/journal.pone.0042406

Figure Lengend Snippet: Ovaries from immature rats were fixed and adjacent paraffin sections were cut. Glut1, Glut2, Glut3 and Glut4 proteins were localized with corresponding specific antibodies by ABC (avidin -biotin complex) method. Column Glut show positivities and immunoreactivities for Glut1, Glut2, Glut3 and Glut4 respectively by IHC. Note that Glut1–4 were not only detected in granulosa cells, theca cells but also in oocyte. Each independent data came form six ovaries. Magnification, 400×, Bar = 20 µm.

Article Snippet: Mouse monoclonal anti-rat Glut4 was from Cell Signaling (Cell Signaling, USA).

Techniques: Avidin-Biotin Assay

Journal: PLoS ONE

Article Title: The Effect of Gonadotropin on Glucose Transport and Apoptosis in Rat Ovary

doi: 10.1371/journal.pone.0042406

Figure Lengend Snippet: The ovaries were also collected for Glut mRNA analysis by real-time PCR and RT-PCR. The Glut mRNA abundance were normalized by 18S rRNA and β-actin. Although eCG significantly increased Glut1, Glut3 and Glut4 mRNA level, these responses were abrogated by eCG antiserum. Neither eCG alone nor the combination of eCG and eCG antiserum elicited a significant response in the Glut2 mRNA regulation. **, P<0.01.

Article Snippet: Mouse monoclonal anti-rat Glut4 was from Cell Signaling (Cell Signaling, USA).

Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction